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Image Search Results
Journal: The Open Dentistry Journal
Article Title: A Method for Rapid Demineralization of Teeth and Bones
doi: 10.2174/1874210601004010223
Figure Lengend Snippet: The choice of the decalcifier reagent affects β-galactosidase activity. The 3-month-old Dspp-lacZ mouse skulls were fixed with 4% PFA and decalcified with either Formical-4 (a), Immunocal (b), or 0.1M EDTA in PBS (c). Absence of blue staining in the odontoblasts of sections (a) and (b) indicates a lack of β-galactosidase enzyme activity. Only the incisor sections from the skulls decalcified with the EDTA- based demineralization agent preserved the activity, as indicated by the blue product of the β-galactosidase substrate (c).
Article Snippet: The skulls were cut from 3-month-old Dspp-LacZ transgenic mice, and skins were removed and then fixed with 0.25% glutaraldehyde in
Techniques: Activity Assay, Staining